000			02805nam a22002297a 4500
003			OSt
005			20230818124253.0
008			160328b xxu||||| |||| 00| 0 eng d
040			_eNLM
050			_aTHS-00406
100			_aChaudhary, Prakash. _93823
245			_aExtended spectrum beta-lactamase producing klebsiella pneumoniae isolated from urinary tract infected patients.
260			_cc2015.
300			_axiii, 57p.
500			_aThesis Report.
520			_aABSTRACT: Klebsiella pneumoniae, one of the bacterial agents associated with urinary tract infection has been often implicated as a major extended spectrum beta-lactamase (ESBL) producer in last few decades. This study was designed to assess the prevalence of ESBL producing klebsiella pneumoniae in urinary isolates at a tertiary care hospital in Kathmandu, Nepal,from July 2014 to December 2014. One thousand nine hundred eighty six mid-stream urine specimens were collected aseptically from the clinically suspected patients of urinary tract infections attending Capital hospital and research center, Kathmandu. The samples were processed following standard guidelines as recommended by American Society for Microbiology (ASM) and the isolates including Klebsiella spp. were identified using the specific biochemical and sugar fermentation tests recommended by ASM. Antibiotic sensitivity testing was done by modified Kirby-Bauer disk diffusion method and interpreted following Clinical and Laboratory Standards Institute (CLSI) guidelines. klebsiella pneumoniae isolates showing resistance upon initial screening with ceftriaxone (30µg) disc were then confirmed for ESBL production by phenotypic confirmatory disc diffusion test (PCDDT) using ceftazidime (30µg) and ceftazidime+ clavulanic acid (30µg+10µg) and cefotaxime (30µg) and cefotaxime+clavulanic acid (30µg+10µg) disc as per CLSI guidelines. Out of a total 1986 specimens investigated, Escherichia coli was isolated in 309(83.9%) and Klebsiella pneumoniae in 38 (10.3%) cases. Initial screening with ceftriaxone disc revealed 18 isolates of Klebsiella pneumoniae to be resistant. Further testing by PCDDT method confirmed 7 (18.4%) Klebsiella pneumoniae isolates to be ESBL producers. Compared to some earlier studies done in Nepal, higher prevalence of ESBL-producing Klebsiella pneumoniae was observed warranting a national surveillance for routine monitoring of ESBL producing Klebsiella pneumoniae isolates. Keywords: Extended spectrum beta lactamase (ESBL), Klebsiella pneumoniae, Urinary Tract Infection (UTI)
650			_aExtended spectrum beta lactamase (ESBL). _93824
650			_aKlebsiella pneumoniae. _93825
650			_aUrinary tract infection (UTI). _93826
856			_uhttp://nhrc.gov.np/contact/ _yVisit NHRC Library
942			_2NLM _cTR
999			_c2324 _d2324