000			02840nam a22002657a 4500
003			OSt
005			20230816121848.0
008			190603b ||||| |||| 00| 0 eng d
040			_eNLM
060			_aTHS-00480
100			_aGhimire,Sapana. _92232
245			_aEffect of abca1-r219k variant polymorphism in serum lipid parameter in patients under statin therapy visiting Manmohan cardiothoracic vascular and transplant center, Nepal.
260			_cc2018.
300			_axvii,114p.
500			_aThesis Report.
520			_aABSTRACT: Background: Dyslipidemia is one of the major risk factor of coronary artery diseases (CAD). To treat dyslipidemia, the physicians are prescribing various anti-lipidemic drugs, major being statin like atorvastatin, rosuvastatin, etc. The response of statins varies individually which may be due to various genetic polymorphisms. R219K polymorphism of ABCA1 gene is found to have role in the response of statin. Objectives: The aim of this study was to evaluate the effect of R219K polymorphism of ABCA1 gene in the lipid lowering effect of statins in patients of dyslipidemia. Research Method: A prospective study was conducted from December 2017 to November 2018 in cardiology OPD of MCVTC. A total of 88 blood samples were taken from patients with dyslipidemia after taking atorvastatin for 3months. Patients serum samples were analyzed for Lipid profile and compared with lipid value before medication and whole blood were analyzed for R219K SNPs using PCR-RFLP to evaluate its effect on lipid improvement following treatment. Data was then entered into excel sheet and analyzed in SPSS v20.0. Results: R219K polymorphism caused significantly higher degree of reduction in value of serum TG (P=0.05), VLDL (P=0.05), TG/HDL ratio (P<0.05), and TC/HDL ratio (P<0.05) in atorvastatin users. However, it was not associated with reduction of serum TC (P=0.83), HDL-C (0.39) and LDL-C levels (P=0.89). Treatment of dyslipidemia was found to be comparatively better in patient with polymorphism than without polymorphism. Conclusion: The KK genotype of R219K polymorphism of ABCA1 gene was associated with significantly greater degree of reduction of TG, VLDL, TG/HDL ratio, and TC/HDL ratio and subsequently with greater degree of increase in HDL-C following atorvastatin treatment in Nepalese patients with dyslipidemia. Keywords: ATP Binding Cassette Transport protein A1 (ABCA1), R219K polymorphism, Lipid Profile, Statin, PCR-RFLP, Nepal.
650			_aATP Binding Cassette Transport protein A1 (ABCA1). _92233
650			_aR219K polymorphism. _92234
650			_aLipid Profile. _92235
650			_a Statin. _92236
650			_aPCR-RFLP. _92237
650			_aNepal. _9362
856			_uhttp://nhrc.gov.np/contact/ _yVisit NHRC Library
942			_2NLM _cTR
999			_c2727 _d2727