000			02673nam a22002417a 4500
999			_c3131 _d3131
003			OSt
005			20220906184724.0
008			220725b ||||| |||| 00| 0 eng d
060			_aRES-01091
100			_aThapa,Sandeep. _94770
245			_aDistribution and comparison of human papilloma virus genotype in cervical cancer in ecological region of Nepal.
260			_cNA.
300			_a13p.
500			_aResearch Report.
520			_aBackground: Cervical cancer is the second leading cause of cancer related deaths, next only to breast cancer worldwide, but is easily preventable if detected early. It accounts for more than 270000 deaths annually and developing countries bear the burden of almost 85% of these cases. Campaign for cervical cancer screening has been carried out in Nepal since many years but it still stands in the world with respect to morbidity and mortality associated with cervical cancer. Method: After obtaining written informed consent, sociodemographic details including clinical history were obtained from the participants using a structured questionnaire. A speculum examination was carried out and cervical cells were collected and a conventional smear were prepared for cytological examination. Further, molecular examination was performed for the molecular characterization. Report: In this study, most of the patients belonged to the age group of 20-25 years and 30-35 years marking 19.67% which is trailed by 35-40 years (18.03%). Likewise, high prevalence of early marriage was reported with an average age of 17 years. Most of the patients experienced abdominal pain (28.6%) followed by chronic backpain (22.85%), and weakness (18.03%). In addition, only 15(12.29%) of women were screened positive on Pap smear. However, out of 107 Pap negative samples, 8 (7.47%) ware reported positive with molecular examination. Among them, five samples were stated with HPV 18 and three with HPV 16. Discussion: It has been well documented in recent years that providing quality assured and effective cervical cytology screening is a challenging task and cytology screening programs have been less successful in reducing cervical cancer. Moreover, this study will be useful for and guide eventual national scale up of HPV testing as a primary screening test in future. Keywords: Cervical cancer, molecular diagnosis, Pap smear, polymerase chain reaction, Nepal
650			_a Cervical cancer. _9341
650			_aMolecular diagnosis. _94771
650			_aPap smear. _9343
650			_aPolymerase chain reaction. _94772
650			_aNepal. _9362
856			_uhttp://nhrc.gov.np/contact/ _yVisit NHRC Library
942			_2NLM _cRR