rdf-syntax-grammar
mods:
  schema location: http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-2.xsd
  title info:
    title: Phenotypic characterization of Staphylococcus aureus isolated from skin and soft tissue infections
  name:
    name part: Shrestha, Suman.
    role:
      role term: creator
  origin info:
    place:
      place term: xxu
    date issued: c2014
  language:
    language term: eng
  physical description:
    extent: xvi, 62p.
  abstract: ABSTRACT: Staphylococcus aureus, being the major cause of mortality and morbidity have been growing burden on health care systems. This study was designed to determine prevalence of S. aureus including methicillin resistant S. aureus (MRSA) isolates in skin and soft tissue infections (SSTIs) along with their phenotypic characterization. S. aureus were isolated and identified following standard microbiological method. Antibacterial susceptibility test was performed by disk diffusion, for oxacillin and vancomycin minimum inhibitory concentration determined by broth micro dilution method and for vancomycin by E-test strips. β lactamse was detected by three different methods viz. clover leaf technique, iodometric and acidometric test in all isolates. Clover leaf technique, microbiological method was considered the gold standard, then the sensitivity, specificity, positive and negative predictive values of the iodometric and acidometric tests were calculated. A total of 108 S. aureus isolates were analyzed in between April and September, 2014. Of these isolates, 40.7% were MRSA which include 22.73% of homogeneous MRSA and 77.27% of heterogeneous MRSA. Of the 44 MRSA isolates, 11 were non-multiresistant oxacillin resistant Staphylococcus aureus (NORSA), defined as being resistant to two or fewer antibiotics other than beta-lactams. Thirty-three were multiresistant oxacillin resistant Staphylococcus aureus (MORSA) strains. D test showed that inducible macrolide-lincosamide-steptogramin-B (MLSB) resistance was 14.8%. Resistance to clindamycin and erythromycin were not associated with MRSA (p>0.05). Iodometric method was found to be more sensitive (92.75%) and had high negative predictive value (86.79%), whereas, acidometric test was more specific (87.18%) and had high positive predictive value (92.19%). MRSA infections are still one of the most threatening infections in the hospitals. Therefore, regular surveillance of MRSA must be carried out in all hospital settings. D te…
  note: Thesis Report.
  subject:
    topic: Staphylococcus aureus
  subject:
    topic: Methicillin resistant S. aureus
  subject:
    topic: Non-multi resistant Oxacillin Resistant S. aureus
  subject:
    topic: Multi Resistant Oxacillin Resistant S. aureus
  subject:
    topic: β lactamase
  subject:
    topic: Inducible Macrolide Lincosamide
  subject:
    topic: Streptogram B
  classification: THS-00377
  identifier: http://nhrc.gov.np/contact/
  record info:
    record creation date: 150716
    record change date: 20220906184645.0