Isolation of salmonella spp. in different meat samples of Kathmandu Valley.

ABSTRACT: The present study was conducted at Central Veterinary Laboratory, Tripureshwor, Kathmandu from Ashoj to Mangsir, 2065 with the aims of determination of the isolation of Salmonella spp. in meat samples of Kathmandu Valley. Altogether 250 meat samples including Goat (75), Chicken (125), and...

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Main Author: Majagaiya, Srijana Pokhrel
Format: Unknown
Language:English
Published: c2009.
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Summary:ABSTRACT: The present study was conducted at Central Veterinary Laboratory, Tripureshwor, Kathmandu from Ashoj to Mangsir, 2065 with the aims of determination of the isolation of Salmonella spp. in meat samples of Kathmandu Valley. Altogether 250 meat samples including Goat (75), Chicken (125), and Buffallo (50) were collected from different retail shops of Kathmandu Valley. All these samples were processed using standard procedures for the isolation and identification of Salmonella spp. from meat samples. All Salmonella isolates were tested to different antibiotics using standard protocol for the determination of antibiotic susceptibility profile. The isolation of salmonella spp. detected case was found to be 9.2%. The detection rate was found highest in the month of Ashoj (11.11%). The isolation of Salmonella spp detected case in retail shops of Kathmandu (11.01%) was higher than that of Bhaktapur (8.77%) and Lalitpur (6.66%). The isolation of Salmonella spp detected in chicken (11.2%) was higher than buffalo (8%) and goat (6.66%) meat samples. Among 23 isolates of Salmonella, 39.13% were S. gallinarum, 34.78% were S. pullorum and 26.08% were Salmonella spp. belonging to Serogroups D and E. Among these serogroups, Salmonella belonging to serogroup D was the most predominant. Multidrug- resistant (MDR) was observed in only 4.34% of the Salmonella isolates. However, most of the isolates in addition to those pathogenic to human (i.e , belonging to Serogroup D and E) were resistant to the antibiotics Tetracycline and Nalidxic acid. All isolates were sensitive to Cephotaxime, Ciprofloxacin, Ofloxacin, and Chloramphenicol. Key words : Salmonella, Serogroups, Multidrug-resistant, Pathogenic, Antibiotics.
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