Immunohistochemical study of Tuberculous Lymphadenitis

This study was conducted at Patan hospital, during September 2002 to March 2003 in joint collaboration with Central Department of Microbiology, Tribhuvan University with the objective to evaluate different staining techniques for the diagnosis of tuberculous lymphadenitis on clinically suspected ca...

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Main Authors:	Nepal Health Research Council (NHRC), Pokharel, Smritee
Format:	Technical Report
Language:	en_US
Published:	Nepal Health Research Council 2013
Subjects:	Immunohistochemical study of Tuberculous Lymphadenitis
Online Access:	http://103.69.126.140:8080/handle/123456789/428
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spelling	oai:103.69.126.140:123456789-4282023-01-04T09:21:02Z Immunohistochemical study of Tuberculous Lymphadenitis Nepal Health Research Council (NHRC) Pokharel, Smritee Immunohistochemical study of Tuberculous Lymphadenitis This study was conducted at Patan hospital, during September 2002 to March 2003 in joint collaboration with Central Department of Microbiology, Tribhuvan University with the objective to evaluate different staining techniques for the diagnosis of tuberculous lymphadenitis on clinically suspected cases. Altogether, 40 biopsies collected at Department of Pathology, Patan Hospital were further analyzed.40 biopsy specimen were stained with Haematoxylin - Eosin stain and Acid fast stain where as 20 biopsies were stained with CD3/ CD20/ S-100 stains respectively and the biopsy cell/tissue features were analyzed. Among 40 cases of suspected tuberculous lymphadenitis cases, 100% cases show edtuberculosis positive in Haematoxylin-Eosin stain. Where as Acid Fast Bacillicould be detected in only 10% of the cases. Greater prevalence of tuberculous lymphadenitis was observed between 20-30 years of age with higher percentage of female’s involvement. Frequency of Cervical and axillary nodes involvement were higher than other. In 57.5% ofcases, right cervical nodes and in 20% of the cases, axillary nodes were found involved. Multiple nodes involvement was observed in 80% of the cases and bilateral nodes in only20% of the cases. CD3 cells were present in higher numbers than CD20 cells. CD3 cells were confined to paracortical areas where as higher CD 20 cells were found in follicular area. Butdue to migratory nature of CD20 cells, they were also found inother parts of lymph nodes. Immunohistochemical staining techniques, though specific, H-E staining and AFB staining in combination still remains a method of choice for the diagnosis of tuberculous lymphadenitis,in developing country like Nepal, because of cost benefit and availability of immunohisto-chemical staining reagents. 2013-01-07T20:17:21Z 2022-11-08T10:14:55Z 2013-01-07T20:17:21Z 2022-11-08T10:14:55Z 2004 Technical Report http://103.69.126.140:8080/handle/123456789/428 en_US application/pdf Nepal Health Research Council
institution	My University
collection	DSpace
language	en_US
topic	Immunohistochemical study of Tuberculous Lymphadenitis
spellingShingle	Immunohistochemical study of Tuberculous Lymphadenitis Nepal Health Research Council (NHRC) Pokharel, Smritee Immunohistochemical study of Tuberculous Lymphadenitis
description	This study was conducted at Patan hospital, during September 2002 to March 2003 in joint collaboration with Central Department of Microbiology, Tribhuvan University with the objective to evaluate different staining techniques for the diagnosis of tuberculous lymphadenitis on clinically suspected cases. Altogether, 40 biopsies collected at Department of Pathology, Patan Hospital were further analyzed.40 biopsy specimen were stained with Haematoxylin - Eosin stain and Acid fast stain where as 20 biopsies were stained with CD3/ CD20/ S-100 stains respectively and the biopsy cell/tissue features were analyzed. Among 40 cases of suspected tuberculous lymphadenitis cases, 100% cases show edtuberculosis positive in Haematoxylin-Eosin stain. Where as Acid Fast Bacillicould be detected in only 10% of the cases. Greater prevalence of tuberculous lymphadenitis was observed between 20-30 years of age with higher percentage of female’s involvement. Frequency of Cervical and axillary nodes involvement were higher than other. In 57.5% ofcases, right cervical nodes and in 20% of the cases, axillary nodes were found involved. Multiple nodes involvement was observed in 80% of the cases and bilateral nodes in only20% of the cases. CD3 cells were present in higher numbers than CD20 cells. CD3 cells were confined to paracortical areas where as higher CD 20 cells were found in follicular area. Butdue to migratory nature of CD20 cells, they were also found inother parts of lymph nodes. Immunohistochemical staining techniques, though specific, H-E staining and AFB staining in combination still remains a method of choice for the diagnosis of tuberculous lymphadenitis,in developing country like Nepal, because of cost benefit and availability of immunohisto-chemical staining reagents.
format	Technical Report
author	Nepal Health Research Council (NHRC) Pokharel, Smritee
author_facet	Nepal Health Research Council (NHRC) Pokharel, Smritee
author_sort	Nepal Health Research Council (NHRC)
title	Immunohistochemical study of Tuberculous Lymphadenitis
title_short	Immunohistochemical study of Tuberculous Lymphadenitis
title_full	Immunohistochemical study of Tuberculous Lymphadenitis
title_fullStr	Immunohistochemical study of Tuberculous Lymphadenitis
title_full_unstemmed	Immunohistochemical study of Tuberculous Lymphadenitis
title_sort	immunohistochemical study of tuberculous lymphadenitis
publisher	Nepal Health Research Council
publishDate	2013
url	http://103.69.126.140:8080/handle/123456789/428
work_keys_str_mv	AT nepalhealthresearchcouncilnhrc immunohistochemicalstudyoftuberculouslymphadenitis AT pokharelsmritee immunohistochemicalstudyoftuberculouslymphadenitis
_version_	1761501121332903936

Immunohistochemical study of Tuberculous Lymphadenitis

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