Screening and confirmatory tests for esbl producing organisms (escherichia coli and klebsiella species) and their genetic diversity through pcr technique

Subedi,Surya.

Screening and confirmatory tests for esbl producing organisms (escherichia coli and klebsiella species) and their genetic diversity through pcr technique - c2012. -

Thesis Report.

SUMMARY: Out of the 580 urine samples processed 89 of the cases were E. coli and Klebsiella sps. Among them 77 were E. coli, 10 were Klebsiella pneumonia and 2 were Klebsiella oxytoca. 86.5% among positive cases were caused by E. coli and found to be 96.1% MDR. Among 89 positive cases 11.23 % was Klebsiella pneumonia and 90% MDR. 2.24% among positive samples was K. oxytoca and found to be 100% MDR. In urine 70.78% of organisms are ESBL positive. Most effective drug was Gentamycin, Amakicin, and Imipenem. In pus and sputum among 221 samples 17 were E. coli and Klebsiella sps. 9 samples were E. coli, 7 were Klebsiella pneumonia and one was Klebsiella oxytoca. All organisms isolated were MDR among which 52.9% were ESBL positive. One isolates was total drug resistant. No one single drug was found effective. Gentamycin, Amakicin and Imipenem were found to be effective comparatively.

Keywords : E.coli, Klebsiella, MDR, ESBL


E.coli.
Klebsiella.
MDR.
ESBL.

THS-00309

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