MARC details
| 000 -LEADER |
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| fixed length control field |
03407 a2200265 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20220906184645.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
150716b xxu||||| |||| 00| 0 eng d |
| 060 ## - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
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| Classification number |
THS-00377 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Shrestha, Suman. |
| 9 (RLIN) |
1596 |
| 245 ## - TITLE STATEMENT |
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| Title |
Phenotypic characterization of Staphylococcus aureus isolated from skin and soft tissue infections. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Date of publication, distribution, etc. |
c2014. |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xvi, 62p. |
| 500 ## - GENERAL NOTE |
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| General note |
Thesis Report. |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
ABSTRACT: Staphylococcus aureus, being the major cause of mortality and morbidity have been growing burden on health care systems. This study was designed to determine prevalence of S. aureus including methicillin resistant S. aureus (MRSA) isolates in skin and soft tissue infections (SSTIs) along with their phenotypic characterization. S. aureus were isolated and identified following standard microbiological method. Antibacterial susceptibility test was performed by disk diffusion, for oxacillin and vancomycin minimum inhibitory concentration determined by broth micro dilution method and for vancomycin by E-test strips. β lactamse was detected by three different methods viz. clover leaf technique, iodometric and acidometric test in all isolates. Clover leaf technique, microbiological method was considered the gold standard, then the sensitivity, specificity, positive and negative predictive values of the iodometric and acidometric tests were calculated. A total of 108 S. aureus isolates were analyzed in between April and September, 2014. Of these isolates, 40.7% were MRSA which include 22.73% of homogeneous MRSA and 77.27% of heterogeneous MRSA. Of the 44 MRSA isolates, 11 were non-multiresistant oxacillin resistant Staphylococcus aureus (NORSA), defined as being resistant to two or fewer antibiotics other than beta-lactams. Thirty-three were multiresistant oxacillin resistant Staphylococcus aureus (MORSA) strains. D test showed that inducible macrolide-lincosamide-steptogramin-B (MLSB) resistance was 14.8%. Resistance to clindamycin and erythromycin were not associated with MRSA (p>0.05). Iodometric method was found to be more sensitive (92.75%) and had high negative predictive value (86.79%), whereas, acidometric test was more specific (87.18%) and had high positive predictive value (92.19%). MRSA infections are still one of the most threatening infections in the hospitals. Therefore, regular surveillance of MRSA must be carried out in all hospital settings. D tests should be made mandatory in all S. aureus isolates as inducible MLSB resistance cannot be detected in routine susceptibility test unless erythromycin and clindamycin are placed 15-26 mm apart. These findings suggest the need of β lactamase test prior to the antibiotic prescription. Key words: - Staphylococcus aureus, Methicillin resistant S. aureus, Non-multi resistant Oxacillin Resistant S. aureus, Multi Resistant Oxacillin Resistant S. aureus, β lactamase, Inducible Macrolide Lincosamide Streptogram B |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Staphylococcus aureus. |
| 9 (RLIN) |
3090 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Methicillin resistant S. aureus. |
| 9 (RLIN) |
3091 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Non-multi resistant Oxacillin Resistant S. aureus. |
| 9 (RLIN) |
3092 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Multi Resistant Oxacillin Resistant S. aureus. |
| 9 (RLIN) |
3095 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
β lactamase. |
| 9 (RLIN) |
3106 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Inducible Macrolide Lincosamide. |
| 9 (RLIN) |
3107 |
| 650 ## - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Streptogram B. |
| 9 (RLIN) |
3108 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Uniform Resource Identifier |
<a href="http://nhrc.gov.np/contact/">http://nhrc.gov.np/contact/</a> |
| Link text |
Visit NHRC Library |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
National Library of Medicine |
| Koha item type |
Thesis Report |
