Modulation of chondrogenesis by DG11 (Genkwadaphnin) in clonal mouse embryonic ATDC5 cells.

Thesis Report.

ABSTRACT: Endochondral bone formation occurs when mesenchymall cells condense to differentiate into chondrocytes- the primary cell types of cartilage. To identify novel factors that are involved in the control of chondrogensis, we investigated the role of DG 11 to induce chondrogenic diffeerentitation of ATDC5 cells. Accumulation of fcartilage nodules due to chondrocytes differentiation and expression of different chondrocyte- specific gene markers such as type 11 collagen, type X collagen, aggrecan, Sox9, Runx2 and Osterix was observed upon treatment of ATDC5 cells with DG11 in conecentration and time dependent manners. Furthermore, phosphorylation of ERK and JNK but not that of p38 MAP kinase reveals stimulatory effect of DG11 for chondrogenic differentiation. Conversely, induction of chondrogenesis was significantly suppressed by treating ATDC5 cells with PD98059 and SP 600125, potent inhibitors of ERK and JNK MAP kinase, respectively. From the above findings, we assumed the possible therapeutic effect of DG11 against various growth disorders such as dwarfism.

Eng.